Detection of Somaclonal Variation in Potato Callus Induced by Uv-c Radiation Using Rapd-pcr
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چکیده
In this study, calli were obtained from in vitro grown potato stem or leaf segments cv. Cosima on MS medium containing 2,4-D, NAA, kinetin and yeast extract. After three subcultures, well grown calli were exposed to UV-C radiation with approximately 6.24 μmol photon/m2/s for 30 min three times at an interval of 2 weeks. Somaclonal variation induced by UV-C radiation was revealed by 5 out of 28 random primers and also ISSR4 primer after PCR amplification of DNA. UV-C radiation changed DNA patterns as a source of genetic variation. However, somaclonal variation can be used for selection of potato calli toward desirable traits, such as salt or drought stress.
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تاریخ انتشار 2006